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af700 conjugated muc5ac  (Novus Biologicals)


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    Novus Biologicals af700 conjugated muc5ac
    Immunofluorescence (IF) of small airway epithelial population at the epithelial level (a) and at the epithelial-endothelial junction (c), (d). (a) DAPI (blue), α-tubulin (green), <t>MUC5AC</t> (yellow), CK5 (magenta). (b) xyz-view of DAPI (blue), α-tubulin (green), MUC5AC (yellow), and CK5 (magenta) co-stain to show pseudostratified columnar epithelium structure of the small airway layer. (c) maximum projection of epithelial layer only. α-tubulin (green), ACE2 (magenta) along the surface/higher z-stacks of small airway epithelium. (d) maximum projection at vascular layer. DAPI (blue), CD31 (yellow), ACE2 (magenta) along vasculature layer/lower z-stacks of small airway-capillary constructs. (e) live perfusion of small airway-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrows indicate the entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.
    Af700 Conjugated Muc5ac, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af700+conjugated+muc5ac/MUC5AC+Antibody+(45M1)+%5BAlexa+Fluor%C2%AE+700%5D/pmc10053540-167-5-7
    Average 91 stars, based on 3 article reviews
    af700 conjugated muc5ac - by Bioz Stars, 2026-10
    91/100 stars

    Images

    1) Product Images from "Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform"

    Article Title: Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform

    Journal: Biofabrication

    doi: 10.1088/1758-5090/ac32a5

    Immunofluorescence (IF) of small airway epithelial population at the epithelial level (a) and at the epithelial-endothelial junction (c), (d). (a) DAPI (blue), α-tubulin (green), MUC5AC (yellow), CK5 (magenta). (b) xyz-view of DAPI (blue), α-tubulin (green), MUC5AC (yellow), and CK5 (magenta) co-stain to show pseudostratified columnar epithelium structure of the small airway layer. (c) maximum projection of epithelial layer only. α-tubulin (green), ACE2 (magenta) along the surface/higher z-stacks of small airway epithelium. (d) maximum projection at vascular layer. DAPI (blue), CD31 (yellow), ACE2 (magenta) along vasculature layer/lower z-stacks of small airway-capillary constructs. (e) live perfusion of small airway-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrows indicate the entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.
    Figure Legend Snippet: Immunofluorescence (IF) of small airway epithelial population at the epithelial level (a) and at the epithelial-endothelial junction (c), (d). (a) DAPI (blue), α-tubulin (green), MUC5AC (yellow), CK5 (magenta). (b) xyz-view of DAPI (blue), α-tubulin (green), MUC5AC (yellow), and CK5 (magenta) co-stain to show pseudostratified columnar epithelium structure of the small airway layer. (c) maximum projection of epithelial layer only. α-tubulin (green), ACE2 (magenta) along the surface/higher z-stacks of small airway epithelium. (d) maximum projection at vascular layer. DAPI (blue), CD31 (yellow), ACE2 (magenta) along vasculature layer/lower z-stacks of small airway-capillary constructs. (e) live perfusion of small airway-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrows indicate the entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.

    Techniques Used: Immunofluorescence, Staining, Construct

    Flow cytometric evaluation of cell types in 3D respiratory tissue construct and table with cluster frequencies. (a) bivariate plot of AQP5 and Sp-C in 3D alveoli-capillary construct. (b) bivariate plot of AQP5 and Sp-C on transwell platform, alveolar monoculture. (c) bivariate plot of AQP5 and Sp-C in submerged T-75 alveolar monoculture. (d) bivariate plot of a-tubulin and MUC5AC in 3D small airway-capillary construct. (e) bivariate plot of α-tubulin and MUC5AC on transwell platform, small airway monoculture. (f) bivariate plot of α-tubulin and MUC5AC in submerged, T-75 small airway culture. Grey = negative control; pink – stained sample. (g) bivariate plot of CD31 and vimentin in 3D respiratory construct. (h) bivariate plot of CD31 and vimentin of submerged T-75 monoculture for endothelial cells, fibroblasts and pericyte, contour plots of cell types overlaid. (i) Stacked column graph of cell populations from (a)-(f). VAMC = vascularized alveolar multi-chip; VBMC = vascularized bronchiolar multi-chip; AEC = alveolar epithelial cells; SAEC = small airway epithelial cells; TW = transwell. Blue = endothelial cells; red = fibroblasts; black = pericytes. FlowJo for compensation matrix calculation and gating analysis.
    Figure Legend Snippet: Flow cytometric evaluation of cell types in 3D respiratory tissue construct and table with cluster frequencies. (a) bivariate plot of AQP5 and Sp-C in 3D alveoli-capillary construct. (b) bivariate plot of AQP5 and Sp-C on transwell platform, alveolar monoculture. (c) bivariate plot of AQP5 and Sp-C in submerged T-75 alveolar monoculture. (d) bivariate plot of a-tubulin and MUC5AC in 3D small airway-capillary construct. (e) bivariate plot of α-tubulin and MUC5AC on transwell platform, small airway monoculture. (f) bivariate plot of α-tubulin and MUC5AC in submerged, T-75 small airway culture. Grey = negative control; pink – stained sample. (g) bivariate plot of CD31 and vimentin in 3D respiratory construct. (h) bivariate plot of CD31 and vimentin of submerged T-75 monoculture for endothelial cells, fibroblasts and pericyte, contour plots of cell types overlaid. (i) Stacked column graph of cell populations from (a)-(f). VAMC = vascularized alveolar multi-chip; VBMC = vascularized bronchiolar multi-chip; AEC = alveolar epithelial cells; SAEC = small airway epithelial cells; TW = transwell. Blue = endothelial cells; red = fibroblasts; black = pericytes. FlowJo for compensation matrix calculation and gating analysis.

    Techniques Used: Construct, Negative Control, Staining

    Related Articles

    Construct:

    Article Title: Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform
    Article Snippet: For the 3D alveoli-capillary construct, aquaporin 5 (AQP5-PE, Santa Cruz Biotechnology, sc-514022) and surfactant protein C (SpC-AF647, Santa Cruz Biotechnology, sc-518029) were used to mark for alveolar epithelial cells. .. For the 3D SAEC-capillary construct, AF700-conjugated MUC5AC (Novus Biologicals, NBP2–32732AF700), PE-conjugated cytokeratin 5/8 (Novus Biologicals, NBP2–47824PE), and AF488-conjugated α-tubulin (Cell Signaling Technology, 8058S) were used to identify small airway epithelial cells. .. To identify the vascular cells comprising the microvascular network in the gel chamber and the side lanes, FITC-conjugated vimentin (eBioscience TM , 11–9897-82) and AF700-conjugated CD31 (R&D Systems, FAB3567N-100) were used.



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    Novus Biologicals af700 conjugated muc5ac
    Immunofluorescence (IF) of small airway epithelial population at the epithelial level (a) and at the epithelial-endothelial junction (c), (d). (a) DAPI (blue), α-tubulin (green), <t>MUC5AC</t> (yellow), CK5 (magenta). (b) xyz-view of DAPI (blue), α-tubulin (green), MUC5AC (yellow), and CK5 (magenta) co-stain to show pseudostratified columnar epithelium structure of the small airway layer. (c) maximum projection of epithelial layer only. α-tubulin (green), ACE2 (magenta) along the surface/higher z-stacks of small airway epithelium. (d) maximum projection at vascular layer. DAPI (blue), CD31 (yellow), ACE2 (magenta) along vasculature layer/lower z-stacks of small airway-capillary constructs. (e) live perfusion of small airway-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrows indicate the entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.
    Af700 Conjugated Muc5ac, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af700+conjugated+muc5ac/MUC5AC+Antibody+(45M1)+%5BAlexa+Fluor%C2%AE+700%5D/pmc10053540-167-5-7
    Average 91 stars, based on 1 article reviews
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    af700-conjugated muc5ac nbp2–32732af700  (Novus Biologicals)
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    Immunofluorescence (IF) of small airway epithelial population at the epithelial level (a) and at the epithelial-endothelial junction (c), (d). (a) DAPI (blue), α-tubulin (green), <t>MUC5AC</t> (yellow), CK5 (magenta). (b) xyz-view of DAPI (blue), α-tubulin (green), MUC5AC (yellow), and CK5 (magenta) co-stain to show pseudostratified columnar epithelium structure of the small airway layer. (c) maximum projection of epithelial layer only. α-tubulin (green), ACE2 (magenta) along the surface/higher z-stacks of small airway epithelium. (d) maximum projection at vascular layer. DAPI (blue), CD31 (yellow), ACE2 (magenta) along vasculature layer/lower z-stacks of small airway-capillary constructs. (e) live perfusion of small airway-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrows indicate the entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.
    Af700 Conjugated Muc5ac Nbp2–32732af700, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af700+conjugated+muc5ac/anti+muc5ac++45m1+/pmc10053540-167-2-7
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    af700-conjugated muc5ac nbp2–32732af700 - by Bioz Stars, 2026-10
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    af700-conjugated muc5ac  (Novus Biologicals)
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    Immunofluorescence (IF) of small airway epithelial population at the epithelial level (a) and at the epithelial-endothelial junction (c), (d). (a) DAPI (blue), α-tubulin (green), <t>MUC5AC</t> (yellow), CK5 (magenta). (b) xyz-view of DAPI (blue), α-tubulin (green), MUC5AC (yellow), and CK5 (magenta) co-stain to show pseudostratified columnar epithelium structure of the small airway layer. (c) maximum projection of epithelial layer only. α-tubulin (green), ACE2 (magenta) along the surface/higher z-stacks of small airway epithelium. (d) maximum projection at vascular layer. DAPI (blue), CD31 (yellow), ACE2 (magenta) along vasculature layer/lower z-stacks of small airway-capillary constructs. (e) live perfusion of small airway-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrows indicate the entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.
    Af700 Conjugated Muc5ac, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af700+conjugated+muc5ac/anti+muc5ac++45m1+/pm35166694-126-2-7
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    Immunofluorescence (IF) of small airway epithelial population at the epithelial level (a) and at the epithelial-endothelial junction (c), (d). (a) DAPI (blue), α-tubulin (green), MUC5AC (yellow), CK5 (magenta). (b) xyz-view of DAPI (blue), α-tubulin (green), MUC5AC (yellow), and CK5 (magenta) co-stain to show pseudostratified columnar epithelium structure of the small airway layer. (c) maximum projection of epithelial layer only. α-tubulin (green), ACE2 (magenta) along the surface/higher z-stacks of small airway epithelium. (d) maximum projection at vascular layer. DAPI (blue), CD31 (yellow), ACE2 (magenta) along vasculature layer/lower z-stacks of small airway-capillary constructs. (e) live perfusion of small airway-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrows indicate the entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.

    Journal: Biofabrication

    Article Title: Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform

    doi: 10.1088/1758-5090/ac32a5

    Figure Lengend Snippet: Immunofluorescence (IF) of small airway epithelial population at the epithelial level (a) and at the epithelial-endothelial junction (c), (d). (a) DAPI (blue), α-tubulin (green), MUC5AC (yellow), CK5 (magenta). (b) xyz-view of DAPI (blue), α-tubulin (green), MUC5AC (yellow), and CK5 (magenta) co-stain to show pseudostratified columnar epithelium structure of the small airway layer. (c) maximum projection of epithelial layer only. α-tubulin (green), ACE2 (magenta) along the surface/higher z-stacks of small airway epithelium. (d) maximum projection at vascular layer. DAPI (blue), CD31 (yellow), ACE2 (magenta) along vasculature layer/lower z-stacks of small airway-capillary constructs. (e) live perfusion of small airway-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrows indicate the entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.

    Article Snippet: For the 3D SAEC-capillary construct, AF700-conjugated MUC5AC (Novus Biologicals, NBP2–32732AF700), PE-conjugated cytokeratin 5/8 (Novus Biologicals, NBP2–47824PE), and AF488-conjugated α-tubulin (Cell Signaling Technology, 8058S) were used to identify small airway epithelial cells.

    Techniques: Immunofluorescence, Staining, Construct

    Flow cytometric evaluation of cell types in 3D respiratory tissue construct and table with cluster frequencies. (a) bivariate plot of AQP5 and Sp-C in 3D alveoli-capillary construct. (b) bivariate plot of AQP5 and Sp-C on transwell platform, alveolar monoculture. (c) bivariate plot of AQP5 and Sp-C in submerged T-75 alveolar monoculture. (d) bivariate plot of a-tubulin and MUC5AC in 3D small airway-capillary construct. (e) bivariate plot of α-tubulin and MUC5AC on transwell platform, small airway monoculture. (f) bivariate plot of α-tubulin and MUC5AC in submerged, T-75 small airway culture. Grey = negative control; pink – stained sample. (g) bivariate plot of CD31 and vimentin in 3D respiratory construct. (h) bivariate plot of CD31 and vimentin of submerged T-75 monoculture for endothelial cells, fibroblasts and pericyte, contour plots of cell types overlaid. (i) Stacked column graph of cell populations from (a)-(f). VAMC = vascularized alveolar multi-chip; VBMC = vascularized bronchiolar multi-chip; AEC = alveolar epithelial cells; SAEC = small airway epithelial cells; TW = transwell. Blue = endothelial cells; red = fibroblasts; black = pericytes. FlowJo for compensation matrix calculation and gating analysis.

    Journal: Biofabrication

    Article Title: Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform

    doi: 10.1088/1758-5090/ac32a5

    Figure Lengend Snippet: Flow cytometric evaluation of cell types in 3D respiratory tissue construct and table with cluster frequencies. (a) bivariate plot of AQP5 and Sp-C in 3D alveoli-capillary construct. (b) bivariate plot of AQP5 and Sp-C on transwell platform, alveolar monoculture. (c) bivariate plot of AQP5 and Sp-C in submerged T-75 alveolar monoculture. (d) bivariate plot of a-tubulin and MUC5AC in 3D small airway-capillary construct. (e) bivariate plot of α-tubulin and MUC5AC on transwell platform, small airway monoculture. (f) bivariate plot of α-tubulin and MUC5AC in submerged, T-75 small airway culture. Grey = negative control; pink – stained sample. (g) bivariate plot of CD31 and vimentin in 3D respiratory construct. (h) bivariate plot of CD31 and vimentin of submerged T-75 monoculture for endothelial cells, fibroblasts and pericyte, contour plots of cell types overlaid. (i) Stacked column graph of cell populations from (a)-(f). VAMC = vascularized alveolar multi-chip; VBMC = vascularized bronchiolar multi-chip; AEC = alveolar epithelial cells; SAEC = small airway epithelial cells; TW = transwell. Blue = endothelial cells; red = fibroblasts; black = pericytes. FlowJo for compensation matrix calculation and gating analysis.

    Article Snippet: For the 3D SAEC-capillary construct, AF700-conjugated MUC5AC (Novus Biologicals, NBP2–32732AF700), PE-conjugated cytokeratin 5/8 (Novus Biologicals, NBP2–47824PE), and AF488-conjugated α-tubulin (Cell Signaling Technology, 8058S) were used to identify small airway epithelial cells.

    Techniques: Construct, Negative Control, Staining